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This study’s goal was to develop an intravenous tramadol regimen that would achieve plasma concentrations comparable to those obtained with the highest oral dosage approved in the US (100 mg q6H), yet allow earlier appearances of drug levels within its presumed therapeutic range. Initial PK modeling simulations were employed to identify doses and dosing intervals that would produce comparable concentrations as seen with 100-mg dosage at steady state.

At Worldwide Clinical Trials (WCT), a contract research laboratory, plasma levels of tramadol and its metabolite O-desmethyltramadol were measured using liquid chromatography-tandem mass spectrometry. Plasma analyses were performed on an API 4000 instrument using Analyst software version 1.6.1 from Applied Biosystems/MDS SCIEX of Ontario Canada as well as Thermo Fisher Scientific’s LIMS Version 7.2.0.03 at Waltham MA; we would like to thank Bhasha Desai and Jeff Stark from WCT for their contributions towards bioanalytical and pharmacokinetic analyses at WCT for their contributions during these analyses.

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Plasma concentrations of R-, S-methadone and buprenorphine were measure among MMT participants using HPLC-mass spectrometry. Furthermore, tramadol metabolites were also assesse both plasmatically and urinarily.

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